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Biological Compatibility Profile on Biomaterials for Bone Regeneration
Published on: November 16, 2018
Tissue reaction to sealing materials: different view at biocompatibility
S Ghanaati1, I Willershausen, M Barbeck
1Institute of Pathology, REPAIR-Lab, Johannes Gutenberg University, Langenbeckstr. 1, 55101 Mainz, Germany. ghanaati@uni-mainz.de
European Journal of Medical Research
|December 17, 2010
Summary
GuttaFlow and AHPlus, root canal sealers, showed different biocompatibility. GuttaFlow exhibited better cell survival and tissue integration, while AHPlus demonstrated higher cytotoxicity and macrophage interaction in vivo.
Area of Science:
- Endodontics
- Biomaterials Science
- Cell Biology
Background:
- Root canal sealers' biodegradability outside the root canal is vital for endodontic treatment success.
- Understanding the biological response to commonly used sealers like GuttaFlow and AHPlus is essential.
Purpose of the Study:
- To evaluate the in vitro and in vivo cell and tissue reactions to GuttaFlow and AHPlus.
- To compare the cytotoxicity and tissue integration of these two root canal sealers.
Main Methods:
- In vitro: Incubation of Human Periodontal Ligament Fibroblasts with sealers, assessing cell proliferation and cytotoxicity.
- In vivo: Subcutaneous implantation in Wistar rats, followed by histological analysis of inflammatory response after 6 weeks.
Main Results:
- In vitro, AHPlus showed significantly higher cytotoxicity than GuttaFlow after 96 hours of incubation.
- In vivo, GuttaFlow formed a fibrous capsule with no degradation, whereas AHPlus induced vascularized granulation tissue with macrophage phagocytosis.
Conclusions:
- GuttaFlow demonstrates favorable biocompatibility with minimal tissue reaction and no degradation upon extrusion.
- AHPlus exhibits significant cytotoxicity and is phagocytosed by macrophages, suggesting potential antibacterial properties and self-degradation when extruded beyond the apex.
