Related Experiment Video
Updated: Jun 6, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
[Urinary proteomics: the diagnostic key for urinary tract abnormalities and kidney diseases in children?]
J H H Ehrich1, E Schiffer, J Drube
1Pädiatrische Nieren-, Leber- und Stoffwechselerkrankungen,Kinderklinik, Medizinische Hochschule Hannover, Carl Neuberg Straße 1, 30625 Hannover, Deutschland.
Abstract:
Due to its accessibility and availability, ease of collection, and correlation with physiology and pathology, urine is an attractive source for clinical proteomics/peptidomics in urology and nephrology. Here, we review the published findings of a reproducible, high-resolution method for peptidome analysis of naturally occurring human urinary peptides and proteins - ranging from 0.8 to 17.0 kDA - using samples from renal patients analyzed by capillary electrophoresis coupled to mass spectrometry (CE-MS). CE-MS identified children with urodynamically relevant ureteric junction obstruction, vesicoureteric reflux of grades IV and V, glomerulopathies, tubulopathies, and chronic kidney disease. Our analysis revealed that the incorporation of urinary proteome analysis in the initial evaluation of children with urinary tract abnormalities will avoid side effects of radiological imaging techniques, reduce costs, and increase the quality-adjusted life years in this patient population. CE-MS can be recommended for clinical prospective studies on the analysis of naturally occurring urinary peptides in children with urinary tract diseases.
Related Concept Videos
Urine Studies I: Urinalysis
Imaging Studies II: Ultrasonography
Acute Kidney Injury IV: Diagnostic Studies and Prevention
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care
Microbiota of the Urogenital Tract
Urinary Tract Calculi III: Medical Management
