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Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Published on: November 10, 2021
In vitro synthesis and characterization of bacteriochlorophyll-f and its absence in bacteriochlorophyll-e producing
Hitoshi Tamiaki1, Jun Komada, Michio Kunieda
1Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan. tamiaki@se.ritsumei.ac.jp
Abstract:
Bacteriochlorophyll(BChl)-f which has not yet been found in natural phototrophs was prepared by chemically modifying chlorophyll-b. The retention time of reverse-phase high-performance liquid chromatography of the synthetic monomeric BChl-f as well as its visible absorption and fluorescence emission spectra in a solution were identified and compared with other naturally occurring chlorophyll pigments obtained from the main light-harvesting antenna systems of green sulfur bacteria, BChls-c/d/e. Based on the above data, BChl-f was below the level of detection in three strains of green photosynthetic bacteria producing BChl-e.
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