Related Experiment Video
Updated: Jun 6, 2026

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Disinfection alternatives for contact surfaces and toys at child care centers
Maribel Jimenez1, Celida I Martinez, Cristobal Chaidez
1Centro de Investigacion en Alimentacion y Desarrollo, Food Safety, Culiacan, Mexico.
Insights
Clorox® Anywhere® (CA) effectively reduced bacteria on child care surfaces, outperforming other disinfectants. This highlights the importance of choosing effective disinfectants for infection control in childcare settings.
Area of Science:
- Microbiology
- Environmental Health
- Infection Control
Background:
- Child care surfaces can harbor and spread disease-causing microorganisms.
- Effective disinfection is crucial for preventing microbial transmission in these environments.
Purpose of the Study:
- To evaluate the efficacy of three disinfectants: CITRUS Farm Edition® (CFE), Clorox® GreenWorks™ (CGW), and Clorox® Anywhere® (CA).
- To determine their effectiveness against Salmonella Typhimurium and Staphylococcus aureus on high chair and ball toy surfaces.
Main Methods:
- Bacterial strains (S. Typhimurium and S. aureus) were inoculated onto high chair and ball toy surfaces.
- Disinfectants were applied via spraying.
- Bacteria were recovered using a sponge method for enumeration.
Main Results:
- Clorox® Anywhere® (CA) demonstrated the highest log reduction for both S. aureus (3.92 Log(10)) and S. Typhimurium (3.22 Log(10)) on the high chair.
- Clorox® GreenWorks™ (CGW) achieved reductions of 2.84 Log(10) for S. aureus and 2.12 Log(10) for S. Typhimurium on the high chair.
- CITRUS Farm Edition® (CFE) showed the lowest efficacy, with reductions of 1.42 Log(10) for S. aureus and 1.53 Log(10) for S. Typhimurium on the ball toy.
Conclusions:
- Clorox® Anywhere® (CA) was the most effective disinfectant across both bacterial species and surfaces tested.
- The findings underscore the need to incorporate proven disinfectant efficacy into child care infection control programs.
Abstract:
Child care surfaces are vehicles for disease-causing organisms. Disinfectant procedures prevent microbial dispersion. This study reports the effectiveness of CITRUS Farm Edition® (CFE), Clorox® GreenWorks™ (CGW) and Clorox® Anywhere® (CA) against Salmonella Typhimurium and Staphylococcus aureus inoculated (1 ml of 9Log(10) CFU/ml) on a high chair and ball toy. Disinfectants were sprayed and bacteria recovered from surfaces by sponge method. Exposing an inoculated high chair to CA resulted in the highest reduction of S. aureus (3.92 Log(10)) and S. Typhimurium (3.22 Log(10)). CGW reduced S. aureus and S. Typhimurium by 2.84 and 2.12 Log(10) from the inoculated high chair, while the inoculated ball toy showed a 2.50 and 1.80 Log(10) reduction, respectively. CFE showed the lowest reduction with 1.42 and 1.53 Log(10) of S. aureus and S. Typhimurium from the inoculated ball toy. CA was the best disinfectant no matter which bacteria or surface was analyzed. Emphasis on the effectiveness of disinfectant products is needed to be included in child care center infection control programs.
Related Concept Videos
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Hand hygiene
Hand washing...
Chemical Agents for Microbial Control
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
