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Related Experiment Video

Updated: Jun 5, 2026

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

An efficient Foxtail mosaic virus vector system with reduced environmental risk.

Zun Liu¹, Christopher M Kearney

¹Department of Biology, Baylor University, Waco, TX 76798, USA.

BMC Biotechnology

|December 18, 2010

Summary

This study developed a crippled plant viral vector (FECT) that expresses foreign genes at high levels when co-agroinoculated with a silencing suppressor. This modified Foxtail mosaic virus (FoMV) vector has reduced biohazard potential and replicates only in plants with suppressed silencing.

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Last Updated: Jun 5, 2026

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Area of Science:

Plant biotechnology
Molecular virology
Genetic engineering

Background:

Plant viral vectors enable cost-effective, high-yield protein expression.
Agrobacterium tumefaciens-mediated agroinoculation delivers viral vectors as transgenes.
Deleting viral genes reduces environmental spread and persistence risks.

Purpose of the Study:

To engineer a safer, high-expression plant viral vector by modifying Foxtail mosaic virus (FoMV).
To assess the expression efficiency of the modified vector with and without silencing suppressors.
To evaluate the vector's host range and biohazard potential.

Main Methods:

Deletion of coat protein (CP) and triple gene block (TGB) genetic segments from FoMV to create the FECT vector series.
Co-agroinoculation of FECT vectors with silencing suppressors (p19 or HcPro) in Nicotiana benthamiana.
Analysis of foreign gene (GFP) expression levels and vector replication capabilities.

Main Results:

The FECT vector, with 29% of the genome deleted, showed minimal marker gene expression alone.
Co-agroinoculation with silencing suppressors resulted in high GFP expression (up to 40% TSP) in N. benthamiana.
The FECT/40 variant demonstrated high foreign gene expression and replication in legumes, but low expression in grasses due to agroinoculation efficiency.

Conclusions:

The FECT/40 vector achieves high-level foreign gene expression with significantly reduced biohazard potential.
The modified vector cannot form virions and requires plant silencing suppression for effective replication.
This engineered viral vector offers a safer alternative for pharmaceutical protein production in plants.