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Updated: Jun 5, 2026
Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
Published on: June 19, 2018
[Screening and identification of a novel ovarian specific protein in mouse]
Wei He1, Li-hua Yang, Li-li Sun
1Department of Obstetrics & Gynecology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. anyhewei@yahoo.com.cn
Objective:
To screen and identify the novel ovarian specific proteins to explore the possible mechanisms in the oogenesis and early embryo development.
Methods:
The oocytes of BALB/C mice were collected for 2-D electrophoresis. After reaction with anti-citrulline antibody, three positive spots were selected for mass spectrometry. Based on the biologic informatic analysis, a novel peptide sequence was found and specific primers were designed for cloning. A full-length cDNA was identified by reverse transcription and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Its nucleotide and possible protein sequences were compared with those in the gene bank. And the tissue-specific characteristic of MOP53 was analyzed by Northern blot.
Results:
A novel peptide sequence was found in murine oocytes, it came from a predicted protein with 330 amino acids. The predicted protein had ∼ 53 400 and pI 8.05 with conserved WD40, F-box motif. A full-length cDNA sequence with 1510 bp was cloned and named MOP53. Located at chromosome 9 F2, this new gene was specifically expressed in ovary.
Conclusions:
An ovarian specific protein is found. It may be involved in the process of oogenesis and early embryo development based on its biologic informatic characteristics.
