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Urine and serum analysis of consumed curcuminoids using an IkappaB-luciferase surrogate marker assay
Sivapriya Ponnurangam1, Fadee G Mondalek, Janita Govind
1800 Research Parkway Suite 350, Oklahoma City, OK, 73104, U.S.A. ramar@swaasth.com.
Background:
curcumin metabolites are detectable in body fluids such as serum and urine. We have developed a novel assay that can detect metabolites in such body fluids by measuring their effect on the nuclear factor kappa B/inhibitor of kappa B (NF-κB/IκB) pathway.
Patients And Methods:
fifteen healthy individuals were enrolled in the study and randomly assigned to two groups: control group (five) and curcumin group (ten). The test group ingested 8 g of the curcuminoids (C(3)-Complex) with 16 oz of bottled water. Blood and urine were collected at 0, 4, 8, and 24 h after ingestion. Degradation of the NF-κB/IκB complex was detected by the Genetic Expression and Measurement (GEM) assay using HCT116 cells stably transfected with PGL3-IκB firefly luciferase.
Results:
using our novel GEM assay, the five controls who had not taken curcumin were identified.
Conclusion:
the GEM assay is a very sensitive and accurate non-invasive assay that could be utilized to detect metabolites in body fluids. It could also serve as a tool to determine participants' compliance during clinical research studies.
