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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 5, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Rapid wide-field photon counting imaging with microsecond time resolution.

Klaus Suhling1, Nicolas Sergent, James Levitt

  • 1Department of Physics, King’s College London, Strand, London WC2R 2LS, UK.

Optics Express
|December 18, 2010
PubMed
Summary
This summary is machine-generated.

A new wide-field imaging method uses photon counting and an ultra-fast camera for time-correlated single photon counting. This technique achieves microsecond resolution for time-resolved imaging of luminescent nanoparticles.

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Area of Science:

  • Optics and Photonics
  • Materials Science
  • Nanotechnology

Background:

  • Time-resolved imaging is crucial for understanding dynamic processes in materials.
  • Traditional methods can suffer from limited resolution or photon pile-up.

Purpose of the Study:

  • To introduce a novel wide-field imaging method for time-correlated single photon counting.
  • To demonstrate its application in time-resolved imaging of nanoparticles.

Main Methods:

  • Utilized a photon counting image intensifier coupled to an ultra-fast CMOS camera (40 kHz frame rate).
  • Employed a pulsed excitation source and luminescent samples.
  • Integrated the system on an inverted microscope.

Main Results:

  • Achieved simultaneous determination of photon arrival times across multiple pixels.
  • Obtained microsecond resolution with reduced photon pile-up.
  • Successfully applied the method to image Europium-containing polyoxometalate nanoparticles.

Conclusions:

  • The developed wide-field imaging method offers high temporal resolution and efficiency.
  • This technique enables advanced time-resolved studies of nanomaterials.