Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy
Etienne Shaffer1, Corinne Moratal, Pierre Magistretti
1École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland. etienne.shaffer@epfl.ch
Abstract:
We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Two-Dimensional Microscopy in Microbiology
Three-Dimensional Microscopy in Microbiology
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...


