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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein.
Tania Saxl1, Faaizah Khan, Matteo Ferla
1Diabetes Research Group, King's College London School of Medicine, Guy's Hospital, London, SE1 1UL, UK.
The Analyst
|December 18, 2010
Summary
New fiber-optic biosensors use fluorescent glucose sensors for continuous glucose monitoring. This non-electrochemistry approach shows promise for diabetes management, offering a stable alternative to current methods.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biophysics
Background:
- Continuous glucose monitoring (CGM) is crucial for diabetes mellitus management.
- Existing electrochemical methods face limitations, necessitating alternative technologies.
- Fluorescent biosensors offer a promising non-electrochemical approach for glucose detection.
Purpose of the Study:
- To develop and evaluate fiber-optic biosensors for continuous glucose monitoring.
- To investigate the use of glucose/galactose binding protein (GBP) labeled with the fluorophore Badan.
- To assess the performance of these sensors in terms of sensitivity, stability, and response time.
Main Methods:
- Development of fiber-optic biosensors utilizing GBP-Badan immobilized on NTA-functionalized agarose or polystyrene beads.
- Attachment of GBP-Badan via an oligohistidine-tag to the bead surfaces.
- Measurement of fluorescence lifetime changes in response to glucose using fluorescence lifetime imaging microscopy and time-correlated single-photon counting.
Main Results:
- Fluorescence lifetime of GBP-Badan increased with glucose concentration.
- Apparent dissociation constants (K(d)) were determined as 13 mM (agarose) and 20 mM (polystyrene) at maximal response at 100 mM glucose.
- Demonstrated good working-day stability for the developed biosensors.
Conclusions:
- Fluorescence lifetime fiber-optic glucose sensors based on GBP-Badan are suitable for development as clinical glucose monitors.
- This technology offers a viable non-electrochemical alternative for continuous glucose monitoring.
- Further development could lead to improved diabetes management tools.
