Related Experiment Video
Updated: Jun 5, 2026
Developing a Clinically Relevant Hemorrhagic Shock Model in Rats
Published on: March 22, 2024
[Hemodynamics and aortic tension induced by two septic shock models in rats]
Man-li Xia1, Min Wang, Rong-kui Chai
1Department of Physiology, College of Medicine, Jiaxing University, Jiaxing 314001, China. sunny3643826@163.com
Objective:
To compare the ventricular-dynamic parameters and thoracic aorta tension induced by two septic shock models in rats.
Methods:
Septic shock models were induced by cecal ligation or puncture (CLP) and intraperitoneal injection of lipopolysaccharide (LPS) in rats. The carotid artery was cannulated and connected to a pressure transducer to determine mean arterial blood pressure (MABP). Ventricular dynamic parameters, including heart rate (HR), left ventricular developed pressure (LVDP) and maximal rise/fall velocity of ventricular pressure (± dP/dtmax) were determined. Isolated thoracic rings were mounted on an organ bath and the tension of the vessel was recorded.
Result:
The mortality was 65.2% in CLP shock rats, but no death in LPS shock rats. The MABP and HR of CLP rats were decreased more prominently than those of LPS rats (P < 0.01). Contraction induced by high K(+) (60 mmol/L) or 10⁻⁶ mol/L phenylephrine (PE) in endothelium-intact and endothelium-denuded aortic rings was all attenuated, but in LPS rats it was more prominent (P < 0.01).
Conclusion:
Two rat septic shock models can decrease ventricular-dynamic parameters and vasoconstriction responsiveness of aorta. The ventricular-dynamic parameters decrease more prominently in CLP model, while vasoconstriction responsiveness of aorta changes more in LPS model.
