Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comprehensive analysis of mitophagy-related genes reveals prognostic signatures in breast cancer: based on immune landscapes and treatment target predict.

Frontiers in immunology·2026

Same author

PETScan: score-based genome-wide association analysis of RNA-Seq and ATAC-Seq data.

Bioinformatics (Oxford, England)·2026

Same author

Combined developmental toxicity of organophosphorus flame retardant TCEP and lead on zebrafish.

Ecotoxicology and environmental safety·2026

Same author

Epigenetic Age Acceleration and Hearing Function in US Older Adults.

Ear and hearing·2026

Same author

Treponema pallidum lipoprotein Tp0768 promotes H3K18 Lactylation modification to target PTK2 and enhance endothelial permeability.

International immunopharmacology·2026

Same author

Study on the effects of zirconia restorations in oral prosthodontics on inflammatory factor levels and masticatory function.

Medicine·2026

Search research articles

Related Experiment Video

Updated: Jun 5, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

[Improve PCR-select differential screening kit with digoxin labelled probe].

Jun Xiao¹, Fei Zou, Shao-xi Cai

¹Department of Tropical Medicine, the First Military Medical University, Guangzhou 510515, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology

|December 21, 2010

Summary

This study demonstrates that replacing isotope probes with DIG-labeled probes in the PCR-Select differential screening Kit enhances specificity and avoids radioactive contamination. This method achieves 90% accuracy, offering a safer alternative for gene expression analysis.

More Related Videos

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

Related Experiment Videos

Last Updated: Jun 5, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

Published on: March 9, 2016

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

Published on: May 21, 2015

Area of Science:

Molecular Biology
Biotechnology

Context:

Differential gene expression analysis is crucial for understanding biological processes.
Traditional methods like PCR-Select kits often use isotope probes, posing safety and disposal challenges.
Suppression Subtractive Hybridization (SSH) is a powerful technique for isolating differentially expressed genes.

Purpose:

To evaluate the efficacy of replacing radioactive isotope probes with digoxigenin (DIG)-labeled probes in the PCR-Select differential screening Kit.
To assess the specificity and accuracy of this modified screening method.

Summary:

Differentially expressed sequences identified via SSH were immobilized and probed using DIG-11-dUTP incorporated during PCR.
Hybridization and detection followed standard DIG-labeled probe protocols.
Reverse Northern blot analysis confirmed 90% correspondence for positive hybridization results obtained with the DIG-labeled probe.

Impact:

The DIG-labeled probe system maintains high specificity in differential screening.
This method eliminates the risks associated with isotope pollution, offering a safer and more environmentally friendly alternative.
The modified PCR-Select kit demonstrates complete replaceability of isotope probes for gene screening applications.