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Related Concept Videos

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
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Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces
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Liquid waveguide-based evanescent wave sensor that uses two light sources with different wavelengths.

Jong-Min Lim1, John Paul Urbanski, Jae-Hoon Choi

  • 1National Creative Research Initiative Center for Integrated Optofluidic Systems and Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea.

Analytical Chemistry
|December 21, 2010
PubMed
Summary

This study presents a novel optofluidic evanescent wave sensor using a poly(dimethylsiloxane) (PDMS) elastomer. The sensor utilizes dual-wavelength light to accurately detect analytes and monitor reactions by minimizing noise and enhancing resolution.

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Area of Science:

  • Optofluidics
  • Chemical Sensing
  • Spectroscopy

Background:

  • Optofluidic evanescent wave sensors offer sensitive detection.
  • Poly(dimethylsiloxane) (PDMS) is a versatile material for fabricating microfluidic devices.
  • Evanescent waves enable interaction with analytes at interfaces.

Purpose of the Study:

  • To develop a prototypic optofluidic evanescent wave sensor.
  • To utilize dual-wavelength light for enhanced analyte detection and noise reduction.
  • To enable real-time monitoring of chemical reactions.

Main Methods:

  • Fabrication of a liquid-core/liquid-cladding (L(2)) waveguide using PDMS.
  • Coupling two light sources with different wavelengths into the waveguide.
  • Utilizing the evanescent wave for light-analyte interaction.
  • Measuring differential absorbance at two wavelengths for normalized signal calculation.

Main Results:

  • Demonstrated reduction of external noise effects through normalized absorbance.
  • Achieved enhanced resolution in sample analysis via differential absorption.
  • Showcased the sensor's capability for real-time chemical reaction monitoring.
  • Leveraged slight miscibility at the core-cladding interface for in situ analysis.

Conclusions:

  • The developed optofluidic sensor provides a robust platform for sensitive and selective chemical detection.
  • Dual-wavelength measurement significantly improves signal-to-noise ratio and analytical resolution.
  • The L(2) waveguide sensor is suitable for real-time monitoring of dynamic chemical processes.