Related Experiment Video
Updated: Jun 5, 2026

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
Effect of interface fertilization on biodegradation of polycyclic aromatic hydrocarbons present in nonaqueous-phase
M C Tejeda-Agredano1, S Gallego, J L Niqui-Arroyo
1Instituto de Recursos Naturales y Agrobiología, Consejo Superior de Investigaciones Científicas, Avenida Reina Mercedes, 10, 41012 Sevilla, Spain.
Abstract:
The main goal of this study was to use an oleophilic biostimulant (S-200) to target possible nutritional limitations for biodegradation of polycyclic aromatic hydrocarbons (PAHs) at the interface between nonaqueous-phase liquids (NAPLs) and the water phase. Biodegradation of PAHs present in fuel-containing NAPLs was slow and followed zero-order kinetics, indicating bioavailability restrictions. The biostimulant enhanced the biodegradation, producing logistic (S-shaped) kinetics and 10-fold increases in the rate of mineralization of phenanthrene, fluoranthene, and pyrene. Chemical analysis of residual fuel oil also evidenced an enhanced biodegradation of the alkyl-PAHs and n-alkanes. The enhancement was not the result of an increase in the rate of partitioning of PAHs into the aqueous phase, nor was it caused by the compensation of any nutritional deficiency in the medium. We suggest that biodegradation of PAH by bacteria attached to NAPLs can be limited by nutrient availability due to the simultaneous consumption of NAPL components, but this limitation can be overcome by interface fertilization.
More Related Videos
Related Concept Videos
Microbial Bioremediation of Hydrocarbons
Microbial Bioremediation of Pesticides
Bioremediation
Microbial Bioremediation of Plastics
Bioplastics
Microbial Wastewater Treatment

