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Updated: Jun 5, 2026
Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
Published on: August 1, 2025
Thrombin expression in prostate: a novel finding
Manish Kohli1, Karin Williams, Jorge L Yao
1Department of Oncology, Mayo Clinic, Rochester, Minnesota 55905, USA. kohli.manish@mayo.edu
Introduction:
A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue.
Methods:
Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays.
Results:
(Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05).
Conclusions:
Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.
