Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelin and platelet function.

E H Ohlstein1, B Storer, P Nambi

  • 1Department of Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406.

Thrombosis Research
|March 15, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cone beam computed tomography (CT)-assisted radiologically inserted gastrostomy. Technical considerations and impact on radiation dose.

Clinical radiology·2025
Same author

The prevalence of anxiety disorders in dermatology outpatients: A systematic review and meta-analysis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2023
Same author

Recipient and donor age impact the risk of developing chronic GvHD in children after allogeneic hematopoietic transplant.

Bone marrow transplantation·2016
Same author

A review of percutaneous transhepatic biliary drainage at a tertiary referral centre.

Clinical radiology·2016
Same author

Mid-term follow-up of stenting in chronic mesenteric ischaemia: a review of six cases.

Irish journal of medical science·2013
Same author

Comorbidity burden in patients with chronic GVHD.

Bone marrow transplantation·2013

Endothelin, a vasoconstrictor peptide, did not directly affect human platelet aggregation. However, it potentiated adenosine diphosphate (ADP)-induced aggregation in rabbit and canine platelets under specific conditions.

Area of Science:

  • Cardiovascular Biology
  • Hemostasis and Thrombosis
  • Endocrinology

Background:

  • Endothelin is a newly discovered vasoconstrictor peptide.
  • Platelet function is crucial in hemostasis and thrombosis.
  • Understanding endothelin's role in platelet activity is important for cardiovascular research.

Purpose of the Study:

  • To investigate the effects of endothelin on human, rabbit, and canine platelet function.
  • To determine if endothelin directly induces platelet aggregation or modulates responses to agonists.
  • To examine endothelin's impact on intracellular calcium mobilization in platelets.

Main Methods:

  • Platelet aggregation assays were performed using various agonists (ADP, collagen, epinephrine, arachidonic acid, PGH2, thrombin).

Related Experiment Videos

  • Endothelin was administered at concentrations ranging from 0.01 nM to 1 microM.
  • Intracellular calcium mobilization was measured in Fura2-loaded human platelets.

    • Main Results:

    • Endothelin did not induce platelet aggregation in human, rabbit, or canine platelets.
    • In human platelets, endothelin (0.1 microM) did not alter aggregation responses to agonists.
    • Endothelin potentiated ADP-induced aggregation in rabbit and canine platelets at low ADP concentrations, but not at high concentrations.
    • Endothelin did not mobilize intracellular calcium in human platelets.

    Conclusions:

    • Endothelin does not directly affect human platelet function, including aggregation and calcium mobilization.
    • Endothelin can potentiate platelet aggregation in response to low concentrations of ADP in rabbits and canines.
    • These findings suggest species-specific effects of endothelin on platelet activity, with potential implications for understanding thrombosis in certain species.