Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synchronous wearable ultrasound for early detection of coronary and carotid artery comorbidity.

Science advances·2026
Same author

Oridonin as a novel KDM5C inhibitor alleviates clonal hematopoiesis-induced cardiac aging <i>via</i> H3K4me3-dependent SASP suppression.

Acta pharmaceutica Sinica. B·2026
Same author

3D ECM-inflammation model on a microfluidic chip for neutrophil transmigration from whole blood investigations.

Lab on a chip·2026
Same author

Steaming duration-dependent effects on the <i>in vivo</i> distribution, anti-fatigue activity, and gut microbiota modulation of <i>Polygonatum cyrtonema</i> Hua polysaccharides.

Frontiers in pharmacology·2026
Same author

Tumor volume change rate based on three-dimensional reconstruction as a predictor of pathological response and survival prognosis in esophageal squamous cell carcinoma with neoadjuvant immunochemotherapy.

BMC cancer·2026
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025

Related Experiment Video

Updated: Jun 5, 2026

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
11:22

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

A molecular inversion probe assay for detecting alternative splicing.

Shengrong Lin1, Wenyi Wang, Curtis Palm

  • 1Stanford Genome Technology Center, Department of Biochemistry, Stanford University School of Medicine, Palo Alto, CA USA. kjuneau@stanford.edu

BMC Genomics
|December 21, 2010
PubMed
Summary
This summary is machine-generated.

A new alternative splicing MIP (asMIP) assay offers sensitive, high-throughput monitoring of pre-mRNA splicing. This method accurately quantifies splice junctions, improving upon existing technologies for genomic-scale analysis.

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Related Experiment Videos

Last Updated: Jun 5, 2026

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
11:22

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics

Background:

  • A need exists for sensitive, high-throughput methods to monitor pre-mRNA splicing.
  • Understanding alternative splicing in human cells requires genomic-scale analysis.

Purpose of the Study:

  • To adapt Molecular Inversion Probes (MIPs) for multiplexed capture and quantitation of splice events.
  • To develop a sensitive and accurate assay for measuring pre-mRNA splicing.

Main Methods:

  • Adapted padlock-probe based Molecular Inversion Probes (MIPs).
  • Developed the alternative splicing MIP (asMIP) assay.
  • Quantified individual MIP capture probes using DNA microarrays and high-throughput sequencing.

Main Results:

  • Successfully identified 100% of positive controls.
  • Demonstrated a strong correlation between the asMIP assay and quantitative PCR.
  • Achieved multiplexed capture and quantitation of individual splice events.

Conclusions:

  • The asMIP assay is a sensitive, accurate, and multiplexed method for pre-mRNA splicing measurement.
  • The assay can accommodate high throughput, potentially exceeding 20,000 splice junctions per reaction.
  • This represents a significant advancement over current splicing analysis technologies.