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Stimulation of Notch Signaling in Mouse Osteoclast Precursors
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Cell fate mediators Notch and Twist in mouse mandibular condylar cartilage

Maria J Serrano1, Sarah So, Kathy K H Svoboda

  • 1Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M University Health Sciences Center, Dallas, TX 75246, USA. mserrano@bcd.tamhsc.edu

Archives of Oral Biology
|December 21, 2010
PubMed
Abstract

Insights

Growth factors FGF-2 and TGF-β2 influence gene expression of Notch and Twist in mandibular condylar cartilage (MCC). These findings suggest FGF-2 and TGF-β2 regulate MCC cell differentiation.

Area of Science:

  • Developmental biology
  • Molecular biology
  • Biochemistry

Background:

  • Mandibular condylar cartilage (MCC) is crucial for jaw development.
  • Notch and Twist signaling pathways play roles in cell differentiation.
  • Understanding growth factor regulation of MCC is important for craniofacial development.

Purpose of the Study:

  • To determine the presence of Notch and Twist in MCC.
  • To investigate the effect of FGF-2 and TGF-β2 on Notch and Twist gene expression in MCC.

Main Methods:

  • Immunohistochemistry was used to detect Notch and Twist proteins in embryonic day 17 (E17) mouse MCC.
  • MCC explants from E17 mice were cultured with varying concentrations of FGF-2 or TGF-β2.
  • Quantitative real-time RT-PCR analyzed Notch and Twist gene expression changes.

Main Results:

  • Notch and Twist were localized in the prechondroblastic and chondroblastic layers of MCC.
  • FGF-2 significantly upregulated Notch 1, Twist 1, and Twist 2 gene expression.
  • TGF-β2 exhibited an inhibitory effect on Notch and Twist gene expression.

Conclusions:

  • MCC explants respond to FGF-2 and TGF-β2, affecting Notch and Twist expression.
  • FGF-2 and TGF-β2 may act as regulators of cell differentiation in the bipotent MCC population.
  • Notch and Twist likely mediate the effects of these growth factors in MCC development.

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