Detection of novel guanine nucleotide binding proteins in bovine retinal rod outer segments

In the rod outer segment membranes of the bovine retina at least two members of the small molecular weight guanine nucleotide binding proteins were identified by means of the technique of binding radiolabeled GTP to nitrocellulose Western blots of proteins separated by sodium dodecyl sulphate gel electrophoresis. Such proteins, of 23 and 25 kDa, are able to specifically bind guanine nucleotides after denaturing treatments, and are not labeled by pertussis or cholera toxin-catalyzed ADP-ribosylation. The binding site is specific for GTP.