Related Experiment Video
Updated: Jun 5, 2026

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
Published on: April 5, 2022
Spectrophotometric study of complex formation equilibria in the presence of interference using hard-soft net analyte
Bahram Hemmateenejad1, Mohsen Nekoeinia, Ghodratollah Absalan
1Department of Chemistry, Shiraz University, Shiraz, Iran. hemmatb@sums.ac.ir
Abstract:
In this article, the ability of a new and efficient hard-soft method, previously proposed by our research group, is reported for modeling of the complex formation equilibria in the presence of interferences. This method is based on the net analyte signal (NAS) concept, which is a part of total signal that is directly related to the concentration of the component of interest. It monitors the concentration changes of any chemical species involved in the evolutionary process without requiring any pure spectra or having previous knowledge about the presence of the interferences. The proposed hard-soft method based on net analyte signal (HS-NAS) only needs a chemical model for one of the species involved in the reaction under study. The reliability of the method was examined by applying it to the measured data and spectrum of the known real systems of Fe(2+)-azithromycin and Ca(2+)-tetracycline.
More Related Videos
Related Concept Videos
Complexometric Titration: Ligands
Complexometric Titration: Overview
Atomic Absorption Spectroscopy: Interference
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Extraction: Advanced Methods
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Complexation Equilibria: The Chelate Effect

