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Reg IV protein and mRNA expression in different rat organs
Josip Azman1, Gordana Starcevic Klasan, Danijel Ivanac
1Department of Anesthesiology and Intensive Care, University Hospital Rijeka, Kresimirova 42, Rijeka, Croatia.
Acta Histochemica
|December 21, 2010
Summary
Regenerating Islet IV (Reg IV) protein is expressed in various rat organs, including the pancreas, stomach, and brain. This discovery suggests a potential role for Reg IV in cell growth and survival across different tissues.
Area of Science:
- Molecular Biology
- Cell Biology
- Physiology
Background:
- The Reg IV gene is known in human digestive organs and is linked to cell regeneration, growth, survival, adhesion, and apoptosis resistance.
- The tissue distribution of Reg IV protein in normal rat organs remains largely uncharacterized.
Purpose of the Study:
- To investigate and map the expression and localization of Reg IV protein across structurally and functionally diverse organs in rats.
Main Methods:
- Western blot analysis to detect Reg IV protein expression.
- Reverse transcription-polymerase chain reaction (RT-PCR) to confirm Reg IV mRNA expression.
- Immunohistochemistry to determine the precise localization of Reg IV protein within tissues.
Main Results:
- Reg IV protein was detected in the pancreas, stomach, small intestine, colon, brain, spleen, kidney, and urinary bladder of adult male Wistar rats.
- RT-PCR confirmed the presence of Reg IV mRNA in these organs.
- Immunohistochemistry provided detailed localization data for Reg IV protein.
Conclusions:
- This study provides comprehensive data on the expression and localization of Reg IV protein in multiple rat organs.
- The widespread expression suggests a potentially significant physiological role for Reg IV, possibly as a growth factor, similar to other Reg family members.
