Related Experiment Video
Updated: Jun 5, 2026

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats
Published on: May 23, 2025
Survey of intravitreal injection techniques among retinal specialists in the United States
Amy E Green-Simms1, Noha S Ekdawi, Sophie J Bakri
1Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota 55905, USA.
Purpose:
To describe the intravitreal injection technique practice patterns of retinal specialists in the United States from April 8, 2010 to April 21, 2010.
Design:
Questionnaire survey.
Methods:
All members of the American Academy of Ophthalmology who self-categorized as "Retinal/Vitreous Surgery" were contacted by e-mail to complete an anonymous, 20-question, internet-based survey.
Results:
A total of 765 retinal specialists (44%) responded to the survey. Most respondents wear gloves (58%) and use an eyelid speculum (92%) when performing an intravitreal injection. More than 99% use povidone-iodine preinjection. The majority measure the injection site from the limbus (56%) and inject straight into the vitreous cavity (96%). Most do not displace the conjunctiva (83%). Seventy-two percent routinely assess postinjection optic nerve perfusion, primarily by gross visual acuity measurement (32%). While nearly one third of participants use prophylactic topical antibiotics preinjection, more than two thirds use topical antibiotics postinjection. Forty-six percent perform bilateral simultaneous intravitreal injections. The majority of respondents use a 30-gauge needle for the injection of ranibizumab (78%) and bevacizumab (60%). However, respondents use both a 27- and 30-gauge needle for the injection of triamcinolone acetonide.
Conclusions:
Retinal specialists in the United States participate in a range of techniques for the care before, during, and after intravitreal injections. Further study is needed to elucidate best practice patterns.

