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Updated: Jun 5, 2026

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
Published on: December 16, 2021
The complete microspeciation of arginine and citrulline
1Department of Pharmaceutical Chemistry, Semmelweis University, Research Group of Drugs of Abuse and Doping Agents, Hungarian Academy of Sciences, Budapest H-1092, Hőgyes Endre utca 9, Hungary.
Abstract:
(1)H NMR-pH titrations of arginine, the most basic natural amino acid and citrulline, its neutral counterpart were carried out. Two other closely related auxiliary compounds were also studied. The 8 macroscopic protonation constants were determined. Combining the four datasets in a deductive method, all the 12 microconstants of arginine, the 4 microconstants of citrulline and arginine amide were calculated. An error-propagation analysis and the pH-dependent distribution of the 8 arginine microspecies are provided.
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