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Updated: Jun 5, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
Published on: December 6, 2013
An innovative miniature microbial fuel cell fabricated using photolithography
You-Peng Chen1, Yue Zhao, Ke-Qiang Qiu
1Department of Chemistry, University of Science & Technology of China, Hefei 230026, China.
Abstract:
Recently microbial fuel cells (MFCs) have attracted increasing interests in both environmental and energy fields. Among the various MFC configurations, miniature microbial fuel cell (mini-MFC) has a great potential for the application in medical, communication and other areas because of its miniature volume and high output power density. In this work, a 25-μL single-chamber mini-MFC was fabricated using the photolithography technique. The plate-shaped gold anodic electrode in the mini-MFC showed a higher electrochemical activity than the stripe-shaped one. A biofilm of Shewanella oneidensis MR-1 was formed on the surface of gold electrode in this micro-liter-scale MFCs. As a result, a maximum power density of 29 mW/m(2) and a maximum current density of 2148 mA/m(2) were achieved by this single-chamber mini-MFC.
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