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Related Concept Videos

Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...

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Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
13:21

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Published on: July 21, 2011

Using lanthanoid complexes to phase large macromolecular assemblies.

Romain Talon1, Richard Kahn, M Asunción Durá

  • 1CEA, IBS, F-38054 Grenoble, France.

Journal of Synchrotron Radiation
|December 21, 2010
PubMed
Summary
This summary is machine-generated.

Novel lanthanoid complexes enable solving low-resolution structures of large protein assemblies. The luminescent europium complex successfully determined the structure of a 444 kDa archaeal aminopeptidase, revealing well-defined electron density maps.

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Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
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Fabrication Procedures and Birefringence Measurements for Designing Magnetically Responsive Lanthanide Ion Chelating Phospholipid Assemblies

Published on: January 3, 2018

Area of Science:

  • Crystallography and structural biology
  • Biophysics
  • Inorganic chemistry

Background:

  • Lanthanoid ions possess large anomalous X-ray scattering at their L(III) absorption edge, making them suitable for anomalous diffraction.
  • Lanthanoid complexes have been developed for non-covalent protein binding, aiding in structural determination.
  • Previous studies have utilized these complexes to solve structures of large multimeric proteins.

Purpose of the Study:

  • To report the use of a luminescent europium tris-dipicolinate complex, [Eu(DPA)3]3-, for solving the low-resolution structure of a large protein.
  • To demonstrate the effectiveness of lanthanoid complexes in obtaining well-defined electron density maps from low-resolution data.
  • To explore a new method for structural analysis of large molecular assemblies.

Main Methods:

  • Anomalous diffraction experiments utilizing a luminescent europium tris-dipicolinate complex ([Eu(DPA)3]3-).
  • Structure determination of a 444 kDa homododecameric aminopeptidase (PhTET1-12s) from Pyrococcus horikoshii.
  • Analysis of experimental electron density maps obtained from low-resolution data.

Main Results:

  • The europium complex enabled the determination of the low-resolution structure of the 444 kDa PhTET1-12s aminopeptidase.
  • The experimental electron density map was surprisingly well-defined despite the low resolution of the data.
  • Phases obtained using the lanthanoid complex yielded maps with structural features typically seen in higher-resolution studies.

Conclusions:

  • Lanthanoid complexes, particularly luminescent ones like [Eu(DPA)3]3-, offer a novel approach for solving the structures of large molecular assemblies.
  • This method is effective even when working with low-resolution diffraction data.
  • The technique facilitates the visualization of detailed structural features, advancing structural biology.