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Antiurolithiatic activity of Pinus eldarica medw: fruits aqueous extract in rats
Hossein Hosseinzadeh1, Ali-Reza Khooei, Zahra Khashayarmanesh
1Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. hosseinzadehh@mums.ac.ir
Purpose:
To evaluate the antiurolithiatic activity of Pinus eldarica fruit on induced calcium oxalate nephrolithiasis in rats.
Materials And Methods:
Calcium oxalate nephrolithiasis in rats was induced by administering ethylene glycol 1% for 30 days via drinking water. The prophylactic and therapeutic groups received P. eldarica fruit extract (500 and 1000 mg/kg/day) as well for 30 days and from the 14th day through the end of the experiment, respectively. The following variables were assessed; urine volume, urinary calcium excretion, and crystalluria. Finally, rats' kidneys were histopathologically examined.
Results:
The aqueous extract prophylactic treatment (500 mg/kg/day) increased urinary calcium excretion. Qualitative analysis of crystalluria and histopathologic examination showed that the administered dose of extract prevented stone formation in the kidneys significantly. The prophylactic treatment did not increase urine volume in comparison with ethylene glycol. Stone formation did not decrease in the treatment group.
Conclusion:
This study indicates that P. eldarica fruit extract prevents calcium oxalate deposition, without producing diuresis.