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Phosphate transport in the kidney.

Heini Murer1, Jürg Biber

  • 1Institute of Physiology and Centre of Integrative Human Physiology, University of Zürich, Zürich, Switzerland.

Journal of Nephrology
|December 21, 2010
PubMed
Summary

Mammalian kidneys reabsorb phosphate using sodium-dependent phosphate cotransporters (Na/Pi-cotransporters) located on the apical surface of proximal tubules. Hormones regulate phosphate levels by altering the abundance of NaPi-IIa cotransporters through interactions with NHERF1.

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Area of Science:

  • Nephrology
  • Molecular Biology
  • Physiology

Background:

  • Phosphate reabsorption in mammalian kidneys occurs in proximal tubules.
  • This process is mediated by apical sodium-dependent phosphate cotransporters (Na/Pi-cotransporters) from the SLC 20 and SLC 34 families.
  • Hormonal and metabolic factors regulate renal phosphate reabsorption to meet bodily requirements.

Purpose of the Study:

  • To investigate the acute hormonal regulation of renal phosphate reabsorption.
  • To elucidate the role of NaPi-IIa cotransporter abundance in this regulation.
  • To understand the interaction between NaPi-IIa and NHERF1 in phosphate transport.

Main Methods:

  • Analysis of apical membrane protein abundance in kidney tubules.
  • Investigation of hormonal effects on Na/Pi-cotransporter expression.
  • Studies on the interaction between NaPi-IIa and PDZ-protein NHERF1.

Main Results:

  • Apical abundance of Na/Pi-cotransporters, particularly SLC34A1 (NaPi-IIa), is dynamically regulated.
  • Hormonal factors acutely adjust renal phosphate reabsorption primarily by altering NaPi-IIa abundance.
  • This modulation involves changes in the interaction between NaPi-IIa and the PDZ-protein NHERF1.

Conclusions:

  • The abundance of apical Na/Pi-cotransporters is a key regulatory point for renal phosphate reabsorption.
  • Acute hormonal control of phosphate homeostasis relies on the regulation of NaPi-IIa via its interaction with NHERF1.