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Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

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Related Experiment Video

Updated: Jun 5, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Recent progress in analytical capillary isotachophoresis.

Petr Gebauer¹, Zdena Malá, Petr Boček

¹Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, Brno, Czech Republic.

Electrophoresis

|December 21, 2010

Summary

Capillary Isoelectric Focusing (cIEF) is a vital technique for analyzing trace analytes in complex samples. This review covers recent advancements in cIEF theory, instrumentation, and applications over the past two years.

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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

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Last Updated: Jun 5, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

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Published on: March 2, 2012

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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Area of Science:

Analytical Chemistry
Separation Science

Background:

Capillary Isoelectric Focusing (cIEF) is a critical technique for preseparation and preconcentration of trace analytes.
Its application is particularly important in analyzing complex or diluted biological and environmental samples.

Purpose of the Study:

To provide a comprehensive review of the recent literature on Capillary Isoelectric Focusing.
To cover advancements over the last two years, building upon previous reviews.
To organize the survey into key areas: theory, methodology, instrumentation, techniques, and applications.

Main Methods:

Literature survey of scientific publications from the past two years.
Categorization of reviewed literature into theoretical, methodological, instrumental, and application-based sections.

Main Results:

Identification of emerging trends and significant developments in cIEF.
Compilation of new theoretical insights and methodological improvements.
Overview of novel instrumentation and techniques enhancing cIEF performance.
Summary of diverse applications showcasing the versatility of cIEF.

Conclusions:

Capillary Isoelectric Focusing remains a powerful and evolving tool in analytical chemistry.
Recent advancements continue to expand its capabilities for trace analyte analysis.
The technique's utility in complex sample matrices is well-established and growing.