Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Empagliflozin-pirfenidone dual therapy improves cardiac function and structure in a preclinical two-hit HFpEF model.

Frontiers in pharmacology·2026
Same author

Ultrasound Imaging and Machine Learning for Nondestructive Sensing in Bioreactors.

ACS omega·2025
Same author

Biocompatibility of decellularized spinach leaves.

npj biomedical innovations·2025
Same author

Antibody-Fab and -Fc features promote Mycobacterium tuberculosis restriction.

Immunity·2025
Same author

Noncanonical T cell responses are associated with protection from tuberculosis in mice and humans.

The Journal of experimental medicine·2025
Same author

Chemical genetic interactions elucidate pathways controlling tuberculosis antibiotic efficacy during infection.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jun 5, 2026

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
07:04

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

Published on: March 2, 2012

Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential.

Megan K Proulx1, Shawn P Carey, Lisa M Ditroia

  • 1Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, USA.

Journal of Biomedical Materials Research. Part A
|December 21, 2010
PubMed
Summary

Researchers created fibrin microthreads for delivering human mesenchymal stem cells (hMSCs). These cell-seeded sutures effectively support hMSC viability, proliferation, and differentiation for tissue repair applications.

More Related Videos

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

Related Experiment Videos

Last Updated: Jun 5, 2026

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
07:04

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

Published on: March 2, 2012

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
09:05

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Localized cell delivery is crucial for effective tissue regeneration.
  • Current methods for cell delivery face challenges in efficiency and engraftment.
  • Human mesenchymal stem cells (hMSCs) are promising for therapeutic applications due to their multipotency.

Purpose of the Study:

  • To develop and evaluate fibrin microthreads as a novel scaffold for cell delivery.
  • To assess the ability of fibrin microthreads to support human mesenchymal stem cell (hMSC) attachment, proliferation, and survival.
  • To determine if hMSCs retain their differentiation potential when cultured on fibrin microthreads.

Main Methods:

  • Discrete fibrin microthreads (100 microm diameter) were fabricated.
  • Microthreads were bundled and seeded with 50,000 hMSCs.
  • Cells were cultured for 5 days, with assessments of cell density, viability (LIVE/DEAD assay), proliferation (Ki-67 staining), and differentiation potential (adipogenesis and osteogenesis).

Main Results:

  • Cell density on microthread bundles increased significantly over 5 days, reaching an average of 731 ± 101 cells/mm(2).
  • LIVE/DEAD and Ki-67 assays confirmed high hMSC viability and proliferation.
  • Functional differentiation assays showed that hMSCs maintained their ability to differentiate into adipocytes and osteocytes.

Conclusions:

  • Fibrin microthreads provide a supportive microenvironment for hMSC viability, proliferation, and multipotency.
  • Cell-seeded fibrin microthreads represent a promising platform technology for enhanced localized cell delivery and engraftment.
  • This approach has the potential to improve therapeutic outcomes in tissue repair and regenerative medicine.