Related Experiment Video
Updated: Jun 5, 2026
Cochlear Implant Surgery and Electrically-evoked Auditory Brainstem Response Recordings in C57BL/6 Mice
Published on: January 9, 2019
[The effects of cochleostomy of hearing threshold in animal experiment]
Olimpia Stanisławek-Sut1, Krzysztof Morawski, Kazimierz Niemczyk
1Katedra i Klinika Otolaryngologii WUM. olimpiaset@op.pl
Introduction:
To assess an effect of cochleostomy on hearing threshold in guinea pigs.
Material And Methods:
The authors performed animal experiments using five 3-month-old guinea pigs. Before experiment hearing threshold were evaluated. Surgery involved access to the temporal bone by a post-auricular incision. After a wide opening of the bulla cochleostomy was created (10,000 turn/min, diamond bur of 0.8 mm diameter). Hearing threshold was identified on the basis of presence of wave V in auditory brainstem responses (ABR) for click and frequency-specific stimulation. Also morphology and latency changes for wave V for this stimulation was assessed. Hearing status was evaluated before, just after and 1-, 2-, and 4-weeks after surgery. For surgical procedure and ABR examination all animals were anesthetized with an intramuscular injection of ketamine (50 mg/kg) mixed with xylazine (9 mg/kg) in the supplemental doses. After surgery the animal was treated by antibioticoterapy for 3 days--Enrofloksacyna 0.3 ml subcutaneouly and analgesic--Tolfedine 0.05 mg in second day.
Results:
Four week observation of ABR morphology and hearing thresholds for click and frequency-specific stimulation of 100 dB SPL intensity showed only temporary changes confirming that cochleostomy did not affect cochlear function.
Conclusions:
The correctly performed cochleostomy in guinea pigs did not affect persistently the cochlear function indicating that such an option of CI electrode insertion in patients is safe.
