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Inhibiting adenoid cystic carcinoma cells growth and metastasis by blocking the expression of ADAM 10 using RNA
Qin Xu1, Xiuming Liu, Wantao Chen
1Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Background:
Adenoid cystic carcinoma is one of the most common types of salivary gland cancers. The poor long-term prognosis for patients with adenoid cystic carcinoma is mainly due to local recurrence and distant metastasis. Disintegrin and metalloprotease 10 (ADAM 10) is a transmembrane protein associated with metastasis in a number of diverse of cancers. The aim of this study was to analyze the relationship between ADAM 10 and the invasive and metastatic potentials as well as the proliferation capability of adenoid cystic carcinoma cells in vitro and in vivo.
Methods:
Immunohistochemistry and Western blot analysis were applied to detect ADAM 10 expression levels in metastatic cancer tissues, corresponding primary adenoid cystic carcinoma tissues, adenoid cystic carcinoma cell lines with high metastatic potential, and adenoid cystic carcinoma cell lines with low metastatic potential. RNA interference was used to knockdown ADAM 10 expression in adenoid cystic carcinoma cell lines with high metastatic potential. Furthermore, the invasive and metastatic potentials as well as the proliferation capability of the treated cells were observed in vitro and in vivo.
Results:
It was observed that ADAM 10 was expressed at a significantly higher level in metastatic cancer tissues and in adenoid cystic carcinoma cell lines with high metastatic potential than in corresponding primary adenoid cystic carcinomas and adenoid cystic carcinoma cell lines with low metastatic potential. Additionally, silencing of ADAM 10 resulted in inhibition of cell growth and invasion in vitro as well as inhibition of cancer metastasis in an experimental murine model of lung metastases in vivo.
Conclusions:
These studies suggested that ADAM 10 plays an important role in regulating proliferation and metastasis of adenoid cystic carcinoma cells. ADAM 10 is potentially an important therapeutic target for the prevention of tumor metastases in adenoid cystic carcinoma.
Insights
Disintegrin and metalloprotease 10 (ADAM 10) is highly expressed in metastatic adenoid cystic carcinoma. Inhibiting ADAM 10 reduces cancer cell proliferation and metastasis, suggesting it as a therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Adenoid cystic carcinoma (ACC) is a common salivary gland cancer with poor prognosis due to recurrence and metastasis.
- Disintegrin and metalloprotease 10 (ADAM 10) is implicated in metastasis across various cancer types.
- Understanding ADAM 10's role in ACC is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the association between ADAM 10 expression and the metastatic and proliferative potential of ACC cells.
- To evaluate the impact of ADAM 10 inhibition on ACC cell behavior in vitro and in vivo.
Main Methods:
- ADAM 10 expression was assessed using immunohistochemistry and Western blot in ACC tissues and cell lines.
- RNA interference was employed to knockdown ADAM 10 in high-metastatic ACC cell lines.
- Cell proliferation, invasion, and metastasis were evaluated in vitro and in a murine lung metastasis model.
Main Results:
- ADAM 10 expression was significantly elevated in metastatic ACC tissues and high-metastatic cell lines compared to primary tissues and low-metastatic cell lines.
- Knockdown of ADAM 10 inhibited ACC cell growth and invasion in vitro.
- Silencing ADAM 10 reduced lung metastasis in vivo.
Conclusions:
- ADAM 10 plays a significant role in regulating ACC cell proliferation and metastasis.
- ADAM 10 represents a potential therapeutic target for preventing tumor metastasis in adenoid cystic carcinoma.
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