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Does a tetracycline resistance determinant of class N exist?
1Laboratoire des Staphylocoques et des Streptocoques, Institut Pasteur, Paris, France.
Antimicrobial Agents and Chemotherapy
|July 1, 1990
Summary
The plasmid pMV120 does not confer tetracycline resistance. Researchers found that tetracycline-susceptible bacteria carried plasmids identical to pMV120, suggesting it is a cryptic plasmid and class N tetracycline resistance determinants are nonexistent.
Area of Science:
- Molecular Biology
- Microbiology
- Genetics
Background:
- The plasmid pMV120 was previously identified as carrying a tetracycline resistance (Tcr) determinant belonging to class N.
- Understanding the mechanisms of antibiotic resistance is crucial in clinical and environmental microbiology.
Purpose of the Study:
- To investigate the nature of the tetracycline resistance determinant in plasmid pMV120.
- To determine the existence of class N tetracycline resistance determinants.
Main Methods:
- Plasmid isolation from bacterial clones.
- Restriction enzyme digestion and analysis of plasmid DNA.
- Transformation experiments to assess tetracycline resistance.
Main Results:
- Tetracycline-susceptible bacterial transconjugants were obtained.
- These transconjugants harbored plasmids with restriction enzyme profiles identical to the original pMV120.
- The presence of pMV120 did not confer resistance to tetracycline.
Conclusions:
- The plasmid pMV120 functions as a cryptic plasmid, lacking a functional tetracycline resistance gene.
- The previously proposed class N of tetracycline resistance determinants does not exist based on these findings.