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Related Experiment Videos

[Determination of virus concentration using a minicomputer].

T Smola1, G Specht, H Liebermann

  • 1VEB Friedrich-Loeffler-Institut, Insel Riems, Betrieb des VEB Kombinat Veterinärimpfstoffe Dessau.

Archiv Fur Experimentelle Veterinarmedizin
|January 1, 1990
PubMed
Summary

This study introduces a new physical method for determining virus concentrations using ultracentrifugation and flow photometry, enhanced by minicomputer analysis for improved accuracy and efficiency.

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Area of Science:

  • Biophysical techniques
  • Analytical chemistry
  • Virology

Context:

  • Traditional methods for virus concentration determination relied on graphical evaluation of extinction-time curves using stencil plates.
  • This graphical approach was prone to inaccuracies and time-consuming.
  • A need existed for a more precise and efficient method for quantifying virus particles.

Purpose:

  • To describe a novel physical method for accurate virus concentration determination.
  • To enhance the precision and efficiency of virus quantification using modern computing.
  • To eliminate errors associated with manual data processing in virus analysis.

Summary:

  • A physical method combining ultracentrifugation through a saccharose gradient and flow photometry for virus concentration analysis is presented.

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  • Direct recording and processing of extinction-time curves using a KC 85/1 minicomputer significantly improved accuracy.
  • This digital approach eliminated reading and calculation errors, reducing overall time.

    • Impact:

    • Achieved virus concentration determination with an accuracy of 4-7%.
    • Substantially reduced the time required for virus quantification.
    • Eliminated human error in data processing, leading to more reliable results.