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[Effects of gathering season and three age affect on main active components of Taxus madia]
Xiaojuan Shi1, Zhiming Xu, Shunhua Fu
1Research and Development Center for Natural Medicine, A Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Objective:
To reveal the influence of harvest season and tree age on the content of taxol and 10-DAB III, and provide the basis for the harvest time of Taxus madia.
Method:
Branches and leaves of the labeled 25 three-year-old plants and 25 five-year-old plants were collected every two months from March 26,2009 to January 26, 2010. Taxol and 10-DAB III content of different age and growth season of Taxus in branches were determined by HPLC.
Result:
Taxol and 10-DAB III content were significantly different in different harvesting age. The content of five-year-old plants was significantly higher than that of three-year-old plants. Taxol and 10-DAB III contents were significantly different in different harvesting season, and the highest content of taxol and 10-DAB III was 0.56, 0.32 mg x g(-1), respectively, in May.
Conclusion:
The May is the suitable harvest season for T. madia, but the suitable harvest age need further study which according to the main active component and biomass accumulation.
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