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Published on: August 9, 2019
[Metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide]
Tongtong Xu1, Hui Guo, Bin Wang
1Department of Natural Medicines and State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China. xutong821124@163.com
Objective:
To investigate the metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide.
Method:
Gossypetin-8-O-beta-D-glucuronide was administrated orally at the dose of 250 mg x kg(-1) to healthy rats, then the urine was collected, pre-treated appropriately and detected by HPLC-DAD-MS(n).
Result:
In the rat urine, gossypetin-8-O-beta-D-glucuronide, along with 6 metabolites, were detected, and their structures were elucidated on the basis of reference standards, UV and MS(n) spectra. The metabolic pathways of gossypetin-8-O-beta-D-glucuronide included mainly deglycosylation, glucuronidation and methylation.
Conclusion:
A rapid and sensitive method for the analysis of metabolites in rat urine after orally administrating gossypetin-8-O-beta-D-glucuronide was established and this will be useful for the further metabolism study of gossypetin-8-O-beta-D-glucuronide.
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