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[Rat in vitro plasma-binding percentage determination of sinomenine hydrochloride by using microdialysis method and
1Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China. zyfeng-2006@163.com
Objective:
To assess feasibility of plasma protein-binding percentage by using microdialysis method.
Method:
Sinomenine hydrochloride was selected as model drug. The in vitro rat plasma protein-binding percentage of three kinds of concentration was determinated by using microdialysis sampling tool and HPLC-UV. At the same time the classical study method, such as ultrafiltration method, was also used to determinate the protein-binding percentage to compare the difference between the two methods.
Result:
The in vitro rat plasma protein-binding percentage of sinomenine hydrochloride using microdialysis method was about 26% and kept relative stable at the concentration range. There was no significant difference between the two methods.
Conclusion:
The results shows that the binding percentage is relative low. Microdialysis method is a new method for the study of protein-binding degree.
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