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Updated: Jun 5, 2026

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
Published on: January 10, 2025
Platelet function, activation and apoptosis during and after apheresis.
Rania Bakry1, Douaa Sayed, Hanan Galal
1Transfusion Center, Department of Clinical Pathology, and Flow Cytometry Laboratory, Faculty of Medicine, South Valley University, Qena, Egypt.
Apheresis processing increases platelet activation and phosphatidylserine expression, indicating early cell damage. However, platelet aggregation function remains largely unaffected during storage, suggesting preserved usability of apheresis-derived platelets.
Area of Science:
- Transfusion Medicine
- Hematology
- Cellular Biology
Background:
- Platelets undergo significant changes during processing and storage, including activation, release reactions, and cell death.
- Apheresis, a method for collecting blood components, may negatively impact platelet yield and functional integrity.
Purpose of the Study:
- To investigate the effects of apheresis on platelet activation, apoptosis, and aggregation.
- To assess the correlation between platelet activation/apoptosis markers and aggregation function post-apheresis and during storage.
Main Methods:
- Platelet concentrates from 50 male volunteers were analyzed.
- Platelet activation markers (CD62, CD154) and apoptosis (Annexin V) were measured.
- Platelet aggregation responses to ADP and collagen were assessed.
Main Results:
- A statistically significant increase in platelet activation markers (CD62, CD154) and Annexin V expression was observed during apheresis and storage.
- Platelet aggregation showed a marked decline.
- Correlations between activation/apoptosis markers and aggregation were limited, particularly during apheresis and storage.
Conclusions:
- Apheresis induces initial platelet activation, impacting phosphatidylserine expression.
- Despite activation markers, platelet aggregation function is not significantly impaired during storage.
- Apheresis-derived platelets may retain functional integrity for transfusion purposes.
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