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Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
Published on: August 24, 2015
In vitro effects on platelets irradiated with short-wave ultraviolet light without any additional photoactive reagent
P Sandgren1, F Tolksdorf, W G Struff
1Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital and Karolinska Institutet, Stockholm, Sweden. per.sandgren@karolinska.se
Short-wave ultraviolet light (UVC) effectively reduces pathogens in platelet concentrates. While UVC treatment shows a minor impact on platelet quality, it remains insignificant by current in vitro standards.
Area of Science:
- Transfusion Medicine
- Blood Component Processing
- Pathogen Reduction Technologies
Background:
- Novel short-wave ultraviolet light (UVC) technology THERAFLEX UV-Platelets offers reagent-free pathogen reduction.
- UVC light can reduce pathogens but may affect platelet (PLT) quality.
Purpose of the Study:
- To evaluate the effects of UVC light pathogen reduction on platelet concentrate quality.
Main Methods:
- Buffy-coat-derived platelets were UVC-irradiated in ethyl vinyl acetate bags.
- UVC-treated and untreated platelet units were stored for 7 days on a flat bed agitator.
- In vitro testing assessed platelet counts, metabolism, and activation markers.
Main Results:
- No significant differences in platelet counts or lactate dehydrogenase.
- Increased glucose consumption and lactate production in UVC-treated units by day 7.
- Higher ATP levels in untreated units from day 5; altered shape change and CD62P expression in UVC-treated units.
- Maintained pH >6.8 and optimal swirling in all units.
Conclusions:
- UVC irradiation has a slight, clinically insignificant impact on in vitro platelet quality.
- The THERAFLEX UV-Platelets system appears safe for platelet concentrate processing.
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