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Published on: June 7, 2016
[Expression of angiotensin-II type 1 receptor at1 mRNA in myeloid leukemia]
Min-Yuan Peng1, Lu-Lu Wang, Ting Liang
1Laboratory of Hematology, Central South University, Changsha, Hunan Province, China.
Abstract:
This study was aimed to explore the expression level of angiotensin-II type 1 receptor (AT1) mRNA in bone marrow of myeloid leukemic patients, and its correlation with the proportion of leukemia cells in samples and Hb, WBC, Plt counting in peripheral blood. 51 samples, including 36 AML, 7 CML, and 8 samples of non-malignant hematological diseases as control group were collected. The expression of at1 mRNA was detected by real time-PCR; the expression levels of at1 gene in AML and CML groups were relatively quantitatively analyzed by using 2(-ΔΔCT) and were compared with control group. The results showed that the expression levels of at1 mRNA in AML, CML and control groups were 0.038 ± 0.076, 0.033 ± 0.039, 0.281 ± 0.366, respectively. at1 gene expression in the myeloid leukemic group was significantly lower than that in the control group. The expression level of at1 mRNA in AML was negatively correlated with the proportion of leukemia cells and positively with Hb level in peripheral blood. It is concluded that at1 gene may play a minor role in leukaemogenesis, however, may promote erythropoiesis.
Insights
Angiotensin-II type 1 receptor (AT1) mRNA expression is significantly lower in myeloid leukemia patients compared to controls. AT1 gene expression in acute myeloid leukemia (AML) correlates negatively with leukemia cell proportion and positively with hemoglobin levels.
Area of Science:
- Hematology
- Molecular Biology
- Oncology
Context:
- Myeloid leukemias, including acute myeloid leukemia (AML) and chronic myeloid leukemia (CML), are characterized by abnormal proliferation of myeloid cells.
- The role of the renin-angiotensin system, specifically the angiotensin-II type 1 receptor (AT1), in leukemogenesis is not fully understood.
Purpose:
- To investigate the expression levels of AT1 mRNA in the bone marrow of patients with myeloid leukemia.
- To determine the correlation between AT1 mRNA expression and leukemia cell proportion, as well as peripheral blood parameters (Hb, WBC, Plt) in these patients.
Summary:
- This study analyzed AT1 mRNA expression in bone marrow samples from 36 AML, 7 CML, and 8 control patients using real-time PCR.
- AT1 mRNA levels were significantly lower in both AML (0.038 ± 0.076) and CML (0.033 ± 0.039) groups compared to the control group (0.281 ± 0.366).
- In AML, AT1 mRNA expression showed a negative correlation with leukemia cell proportion and a positive correlation with hemoglobin levels.
Impact:
- The findings suggest that the AT1 gene may play a limited role in the development of myeloid leukemia.
- However, AT1 may potentially promote erythropoiesis, indicating a possible role in red blood cell production within the context of leukemia.
