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Updated: Jun 5, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
Published on: September 1, 2019
PU.1 directly regulates retinoic acid-induced expression of RIG-G in leukemia cells
Zhi-Min Gu1, Chuan-Xu Liu, Shao-Fang Wu
1Department of Pathophysiology and Chemical Biology, Division of Shanghai Universities E-Institutes, Key laboratory of Cell Differentiation and Apoptosis of Minister of Education, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.
Abstract:
RIG-G is a retinoic acid- or interferon-induced gene with potential anti-proliferation function. However, the mechanism underlying ATRA-induced RIG-G induction is not completely understood. Here, we demonstrate that ATRA up-regulates the expression of PU.1, which in turn directly binds to the promoter and increases the expression of RIG-G gene. Luciferase reporter assay and electrophoretic mobility shift assay reveal that PU.1 preferentially binds to one of the two putative binding sites on the RIG-G promoter. Moreover, silencing of PU.1 by shRNA markedly inhibited ATRA- but not IFNα-induced expression of RIG-G. These data provide new insight into the mechanism of ATRA-induced RIG-G expression.
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