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[Determination of rat serum emodin level by microemulsion liquid chromatography with direct sample loading]
Yong Shi1, Bing-jun Wang, Xiao-kai Lin
1Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou, China. shiyong163sy@sina.com
Objective:
To establish a microemulsion liquid chromatography system with direct sample loading for determining the serum level of emodin in rats.
Methods:
The separation was performed on C₁₈ column (Hypersil BDS, 5 µm,150 mm×4.6 mm) with the microemulsion mobile phase consisting of 3.3% (w/V) SDS, 6.6% (V/V) n-butyl alcohol, and 1.0% (V/V) octane and water. The flow rate was 1.0 ml/min and the detection wavelength was 254 nm.
Results:
The linear range of emodin detection was 0.333-5.32 µg/ml. The average recovery was 99.65% with a RSD of 3.60%. The limit of quantification was 0.1386 µg/mL.
Conclusion:
Microemulsion liquid chromatography system with direct sample loading allows simple, accurate and rapid determination of emodin in rat serum.
