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Formation of Muscle Fibers from Myoblasts

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Related Experiment Video

Updated: Jun 5, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
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Published on: May 17, 2016

Connective tissue fibroblasts and Tcf4 regulate myogenesis.

Sam J Mathew1, Jody M Hansen, Allyson J Merrell

  • 1Department of Human Genetics, University of Utah, 15 North 2030 East, Salt Lake City, Utah 84112, USA.

Development (Cambridge, England)
|December 24, 2010
PubMed
Summary

Connective tissue fibroblasts regulate muscle development by influencing fiber type and maturation. This study identifies novel extrinsic and intrinsic mechanisms controlling myogenesis, highlighting connective tissue

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Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
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Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat

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Last Updated: Jun 5, 2026

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Published on: May 17, 2016

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Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

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Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
09:49

Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat

Published on: June 9, 2021

Area of Science:

  • Muscle biology and connective tissue interactions.
  • Developmental biology and cellular regulation.

Background:

  • Muscle and connective tissue are closely linked, implying crucial developmental interactions.
  • Studying muscle connective tissue is challenging due to a lack of specific molecular markers.
  • Transcription factor Tcf4 (Tcf7l2) is identified as a marker for connective tissue fibroblasts.

Purpose of the Study:

  • To investigate the role of connective tissue fibroblasts in myogenesis.
  • To identify molecular mechanisms by which fibroblasts regulate muscle development.
  • To explore novel extrinsic and intrinsic regulators of muscle fiber type and maturation.

Main Methods:

  • Utilized Tcf4(GFPCre) mice for genetic manipulation of connective tissue fibroblasts.
  • Analyzed the impact of fibroblast manipulation on myogenesis, including muscle fiber type and maturation.
  • Investigated Tcf4's role in both fibroblast-dependent and intrinsic myogenic cell regulation.

Main Results:

  • Connective tissue fibroblasts critically regulate muscle fiber type development and maturation.
  • Fibroblasts promote slow myogenesis and the fetal-to-adult muscle transition via Tcf4-dependent signals.
  • A Tcf4-independent mechanism involving fibroblasts promotes the formation of large myofibers.
  • Low levels of Tcf4 in myogenic cells intrinsically promote both slow and fast myogenesis, enhancing fiber type maturation.

Conclusions:

  • Identified novel extrinsic (fibroblast-mediated) and intrinsic (myogenic cell-mediated) mechanisms regulating myogenesis.
  • Demonstrated the critical role of connective tissue fibroblasts as regulators of muscle progenitor niches and myogenesis.
  • Established connective tissue as a vital component for adult muscle structure, function, and developmental regulation.