Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 5, 2026

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

PML protein analysis using imaging flow cytometry.

Lizz Grimwade1, Emma Gudgin, David Bloxham

  • 1Haemato-Oncology Diagnostics Service, Department of Haematology, Addenbrooke's Hospital, Cambridge, UK. lizz.grimwade@addenbrookes.nhs.uk

Journal of Clinical Pathology
|December 24, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of 1- and 2-unit red blood cell transfusions on continuously monitored vital signs: a longitudinal study.

Blood transfusion = Trasfusione del sangue·2026
Same author

Leukaemia Flow Cytometry in Yemen.

International journal of laboratory hematology·2026
Same author

Imaging Flow Cytometry Detection of Cytogenetic Abnormalities in Circulating CD34+ Cells Predicts Leukemic Transformation in Myelofibrosis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same author

Imaging Flow Cytometric Assessment of del(17p) and amp(1q21) in Circulating Plasma Cells of Myeloma Patients.

Current protocols·2026
Same author

Telomere attrition becomes an instrument for clonal selection in aging hematopoiesis and leukemogenesis.

Nature genetics·2025
Same author

Megakaryocyte emperipolesis in myeloproliferative neoplasms: Are neutrophils friends or foes?

Journal of leukocyte biology·2025
Same journal

Measurement of microinvasive breast carcinoma in the 6th edition WHO Classification: risk of overstaging and clinical implications.

Journal of clinical pathology·2026
Same journal

Development and internal validation of a multifactorial risk model for predicting disease-free survival in oral squamous cell carcinoma.

Journal of clinical pathology·2026
Same journal

HER2-low as a unique subgroup in breast cancer: insights from neoadjuvant chemotherapy response and survival analysis.

Journal of clinical pathology·2026
Same journal

Polyp-forming Dieulafoy lesion of the rectum.

Journal of clinical pathology·2026
Same journal

Defining biochemical, pathological and molecular factors prognostic in terms of disease control and survival in high-grade extremity soft tissue sarcoma: a scoping review.

Journal of clinical pathology·2026
Same journal

MILGDF: a multi-task, instance-level supervised model for oral squamous cell carcinoma integrating local-global attention and dynamic decision fusion.

Journal of clinical pathology·2026
See all related articles

Imaging flow cytometry offers a more sensitive and objective method for diagnosing acute promyelocytic leukaemia (APML) compared to traditional immunofluorescence microscopy. This advanced technique enhances diagnostic accuracy for APML and other blood cancers.

Area of Science:

  • Hematology
  • Cellular Biology
  • Diagnostic Technology

Background:

  • Acute promyelocytic leukaemia (APML) diagnosis relies on immunofluorescence microscopy to detect abnormal PML bodies.
  • Current immunofluorescence methods are subjective and have limited sensitivity for APML detection.

Purpose of the Study:

  • To evaluate imaging flow cytometry as a more sensitive and objective alternative to immunofluorescence microscopy for APML diagnosis.
  • To assess the potential of imaging flow cytometry in improving the detection rates of APML.

Main Methods:

  • Bone marrow and peripheral blood cells from 18 acute myeloid leukaemia (AML) patients (including 4 APML cases) were analyzed.
  • Cells were stained with an anti-PML antibody, and data were acquired using an ImageStream imaging flow cytometer.

More Related Videos

IP-FCM: Immunoprecipitation Detected by Flow Cytometry
12:17

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Published on: December 2, 2010

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
08:38

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

Related Experiment Videos

Last Updated: Jun 5, 2026

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

IP-FCM: Immunoprecipitation Detected by Flow Cytometry
12:17

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Published on: December 2, 2010

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
08:38

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

Published on: October 4, 2018

  • The modulation feature of the imaging flow cytometry system was utilized for data analysis.

    • Main Results:

    • Imaging flow cytometry successfully identified cases of APML.
    • The technique demonstrated increased sensitivity compared to conventional immunofluorescence microscopy by analyzing a larger cell population.
    • The potential for incorporating disease-specific antigens was highlighted for enhanced diagnostic capability.

    Conclusions:

    • Imaging flow cytometry is a viable and sensitive alternative for diagnosing APML.
    • This technology offers significant potential for improving the diagnosis of haematological malignancies.
    • Further applications of imaging flow cytometry in clinical diagnostics are anticipated.