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Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative
Andrea K Boggild1, Ana Pilar Ramos, Braulio Mark Valencia
1Tropical Disease Unit, Toronto General Hospital, 200 Elizabeth Street, 13th Floor, North Wing, Room 1350, Toronto, ON M5G 1C4, Canada. andrea.boggild@utoronto.ca
Abstract:
We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for secondarily infected lesions (n = 8) were 100%. In primarily nonulcerative lesions (n = 15), the sensitivity of FPLI PCR was inferior to that of pooled-invasive-specimen PCR (72.7% versus 100%) (P = 0.10). FPLI PCR is sensitive, specific, and unlike invasive procedures, can be used in secondarily infected ulcers. Invasive specimen collection is superior in nonulcerative lesions.
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