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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jun 5, 2026

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
08:40

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

Published on: November 29, 2016

A spatial and temporal map of C. elegans gene expression.

W Clay Spencer1, Georg Zeller, Joseph D Watson

  • 1Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee 37232, USA.

Genome Research
|December 24, 2010
PubMed
Summary
This summary is machine-generated.

Researchers mapped gene expression in C. elegans cells, discovering novel transcriptionally active regions (TARs) and revealing dynamic gene regulation during development and across cell types.

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Last Updated: Jun 5, 2026

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08:40

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10:27

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Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

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Area of Science:

  • Genomics
  • Developmental Biology
  • Molecular Biology

Background:

  • The C. elegans genome is fully sequenced, with detailed single-cell anatomical data available.
  • Understanding gene expression patterns is crucial for deciphering cellular differentiation and organismal development.

Purpose of the Study:

  • To link gene expression profiles to specific cell types in C. elegans.
  • To identify novel transcripts and regulatory elements within the C. elegans genome.
  • To create high-resolution gene expression maps for understanding cellular differentiation.

Main Methods:

  • RNA isolation from specific cells and developmental stages using fluorescence-activated cell sorting (FACS) and mRNA-tagging.
  • Whole-genome gene expression profiling using tiling arrays for over 30 cell types and developmental stages.
  • Machine-learning and self-organizing map analyses for transcript identification and regulatory element discovery.

Main Results:

  • Detection of known transcripts and identification of novel transcriptionally active regions (TARs) in noncoding genomic domains (at least 10% of the genome).
  • Approximately 75% of detected transcripts show differential expression across developmental stages and cell types.
  • Identification of known and novel transcription factor and miRNA-binding sites, suggesting regulatory roles for TARs.

Conclusions:

  • Cell-specific, whole-genome expression profiling provides a powerful strategy for transcript discovery and functional genomics.
  • The identified novel transcripts and regulatory elements offer new avenues for investigating gene function in C. elegans cellular differentiation.
  • High-resolution gene expression maps generated are foundational for future studies on gene roles in development.