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An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

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Polycomb preferentially targets stalled promoters of coding and noncoding transcripts.

Daniel Enderle¹, Christian Beisel, Michael B Stadler

¹Department of Biosystems Science and Engineering, ETH Zurich, CH-4058 Basel, Switzerland.

Genome Research

|December 24, 2010

Summary

This summary is machine-generated.

Polycomb (PcG) and Trithorax (TrxG) proteins maintain gene expression. This study reveals PcG proteins bind novel microRNA gene promoters, expanding their regulatory role to noncoding RNAs.

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Area of Science:

Developmental Biology
Epigenetics
Genomics

Background:

Polycomb (PcG) and Trithorax (TrxG) proteins epigenetically regulate gene expression.
Their precise mechanisms and genome-wide targets remain incompletely understood.

Purpose of the Study:

To comprehensively map Polycomb group and Trithorax binding sites.
To identify novel target genes and regulatory mechanisms of PcG proteins.

Main Methods:

ChIP-seq and RNA-seq in Drosophila S2 cells.
Genome-wide transcription start site (TSS) detection.

Main Results:

Thousands of PcG and TrxG binding sites were identified.
PcG proteins bind stalled promoters of annotated genes and intergenic regions.
Novel PcG binding sites were found at promoters of microRNA genes.

Conclusions:

PcG proteins regulate noncoding RNAs, including microRNAs.
This expands the known scope of Polycomb group protein function in development and cellular processes.