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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
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Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
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Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

Ebolavirus VP35 is a multifunctional virulence factor.

Daisy W Leung1, Kathleen C Prins, Christopher F Basler

  • 1Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA.

Virulence
|December 24, 2010
PubMed
Summary
This summary is machine-generated.

Ebola virus VP35 protein aids viral replication and immune evasion. Structural studies reveal key regions of the VP35 interferon inhibitory domain (IID) that could be targeted for new Ebola virus (EBOV) treatments.

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Area of Science:

  • Virology
  • Structural Biology
  • Immunology

Background:

  • Ebola virus (EBOV) causes severe hemorrhagic fever with no approved treatments.
  • The EBOV VP35 protein is crucial for viral RNA synthesis and immune evasion.

Purpose of the Study:

  • To elucidate regulatory mechanisms of EBOV VP35.
  • To identify potential therapeutic targets for EBOV countermeasures.

Main Methods:

  • X-ray crystallography to solve VP35 IID structures (free and dsRNA-bound).
  • Biochemical, cell biological, and virological assays.

Main Results:

  • Determined structures of Zaire and Reston EBOV VP35 IID.
  • Identified distinct regions within VP35 IID contributing to immune evasion and RNA synthesis.
  • Demonstrated VP35 IID's role in viral virulence.

Conclusions:

  • EBOV VP35 IID is a multifunctional domain critical for viral pathogenesis.
  • Distinct regions of VP35 IID are potential targets for therapeutic intervention against EBOV.