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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jun 5, 2026

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

Published on: November 26, 2010

Experimental metastasis and CTL adoptive transfer immunotherapy mouse model.

Mary Zimmerman¹, Xiaolin Hu, Kebin Liu

¹Department of Biochemistry and Molecular Biology, Medical College of Georgia, USA.

Journal of Visualized Experiments : Jove

|December 24, 2010

Summary

This study details an experimental metastasis mouse model, ideal for testing therapies targeting lung metastases. The model allows controlled study of tumor cell survival, extravasation, and colonization, aiding metastasis research.

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Area of Science:

Oncology
Immunology
Preclinical Research

Background:

Metastasis is a complex process and a major cause of cancer-related mortality.
Preclinical models are essential for understanding metastasis and developing effective therapies.
Existing models may not fully recapitulate the late stages of spontaneous metastasis.

Purpose of the Study:

To describe and validate a physiologically relevant experimental metastasis mouse model.
To demonstrate the utility of this model for evaluating anti-metastasis therapies.
To establish a reproducible method for studying tumor cell colonization in the lungs.

Main Methods:

Intravenous injection of tumor cells into mouse tail veins to establish lung metastases.
Controlled tumor cell dosage and growth time to modulate metastasis extent.
Intravenous administration of tumor-specific cytotoxic T lymphocytes (CTLs) to assess therapeutic efficacy.
Lung metastasis quantification via ink inflation for enhanced visualization and counting.

Main Results:

The model successfully mimics key steps of spontaneous metastasis: survival in circulation, extravasation, and organ colonization.
Lung metastases formation and burden are controllable by varying injected tumor cell numbers and growth duration.
The model allows for the study of various metastasis stages, from minimal to extensive.
Ink inflation provides a reliable method for observing and quantifying lung metastases.

Conclusions:

The experimental metastasis mouse model is a valuable, simple, and physiologically relevant tool for metastasis research.
This model is ideal for evaluating therapeutic strategies targeting established lung metastases.
The model's controllability and reproducibility support its use in preclinical cancer studies.