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Updated: Jun 5, 2026

Traction Force Microscopy to Study B Lymphocyte Activation
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Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

Imaging techniques for assaying lymphocyte activation in action.

Lakshmi Balagopalan1, Eilon Sherman, Valarie A Barr

  • 1Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Nature Reviews. Immunology
|December 24, 2010
PubMed
Summary

Advanced imaging techniques reveal cellular and molecular details of lymphocyte activation. This review covers methods assessing lymphocyte activation from whole animals to single molecules, aiding in model validation and revision.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Lymphocyte activation is crucial for adaptive immunity.
  • Traditional methods offered limited insight into dynamic cellular processes.
  • Recent technological advancements enable real-time observation of lymphocyte activation.

Purpose of the Study:

  • To review current imaging techniques for studying lymphocyte activation.
  • To discuss the advantages and limitations of various imaging modalities.
  • To highlight how imaging advances have impacted lymphocyte activation models.

Main Methods:

  • Review of literature on imaging techniques applied to lymphocyte activation.
  • Categorization of methods based on scale (whole animal to single molecule).

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  • Discussion of technical aspects like spatial and temporal resolution, and labeling tools.

    • Main Results:

    • Imaging provides unprecedented cellular and molecular detail of lymphocyte activation.
    • Specific imaging techniques have validated, revised, or replaced existing activation models.
    • Methods range from in vivo imaging to in vitro single-molecule studies.

    Conclusions:

    • Imaging techniques are indispensable tools for understanding lymphocyte activation.
    • Continued technological innovation will further refine our knowledge.
    • A comprehensive understanding of method capabilities and limitations is essential for future research.