Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Evidence-based multidisciplinary model of care for newborn screening in spinal muscular atrophy.

Genetics in medicine : official journal of the American College of Medical Genetics·2026
Same author

Surface nanocratering by localized plasma expansion induced by slow highly charged ions.

Physical review. E·2026
Same author

Search for New Physics in Jet Multiplicity Patterns of Multilepton Events at sqrt[s]=13  TeV.

Physical review letters·2025
Same author

Observation of Λ Hyperon Local Polarization in p-Pb Collisions at sqrt[s_{NN}]=8.16  TeV.

Physical review letters·2025
Same author

Search for a Neutral Gauge Boson with Nonuniversal Fermion Couplings in Vector Boson Fusion Processes in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2025

Related Experiment Video

Updated: Jun 5, 2026

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024

Evaluation of delivery conditions for cutaneous plasmid electrotransfer using a multielectrode array.

B Ferraro1, L C Heller, Y L Cruz

  • 1Department of Molecular Medicine, University of South Florida, Tampa, FL, USA.

Gene Therapy
|December 24, 2010
PubMed
Summary

A novel multielectrode array (MEA) enables efficient in vivo electroporation (EP) for large-area skin DNA delivery. This method allows for controlled transgene expression by adjusting voltage and plasmid dose.

More Related Videos

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
04:46

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

Related Experiment Videos

Last Updated: Jun 5, 2026

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro
04:46

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

Published on: September 12, 2011

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Dermatology

Background:

  • Electroporation (EP) is a method for delivering molecules into cells.
  • Current EP methods face challenges in large-surface area tissue delivery.
  • A multielectrode array (MEA) offers a potential solution for enhanced delivery.

Purpose of the Study:

  • To evaluate a multielectrode array (MEA) for in vivo electroporation (EP).
  • To assess the impact of field strength and pulse width on transgene expression.
  • To determine the viability of MEA for cutaneous DNA delivery.

Main Methods:

  • Utilized a guinea pig model for intradermal plasmid DNA injection.
  • Applied EP with a novel MEA design to the skin.
  • Measured luciferase gene expression to quantify delivery efficiency.

Main Results:

  • Increased voltage magnitude and duration correlated with higher luciferase expression.
  • Transgene expression levels were controllable via field strength and plasmid dose.
  • The MEA facilitated efficient plasmid DNA delivery to large skin areas.

Conclusions:

  • The MEA design is effective for cutaneous DNA delivery via in vivo EP.
  • This technology allows for tunable transgene expression.
  • MEA-based EP is a promising approach for large-surface area genetic material delivery to skin.